Method and apparatus for high throughput high efficiency transfection of cells
US11859162B2 · kind B2 · utility
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5References
18Claims
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Key dates
| Filing date | Aug 30, 2019 |
| Grant date | Jan 2, 2024 |
| Priority date | — |
| Expiry date | Aug 12, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.