Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
US11859219B1 · kind B1 · utility
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19Claims
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Key dates
| Filing date | Dec 29, 2017 |
| Grant date | Jan 2, 2024 |
| Priority date | — |
| Expiry date | Mar 1, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are compositions and methods for effecting alterations at a defined location in the genome of a plant cell or plant protoplast. Further disclosed are methods using an RNA-guided nuclease to alter a target nucleotide sequence in a cell or protoplast of a plant; embodiments of such methods include treatments with chemical or physical reagents or treatment of the cell or protoplast with a specific thermal regime, such as a heat treatment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.