Methods and systems and related compositions for mixtures separation with a solid matrix

US11866695B2 · kind B2 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Rustem F. Ismagilov · Altadena, US
• Erik Jue · Pasadena, US
• Daan Witters · Geetbets, BE

Key dates

• Filing date: Dec 22, 2020
• Grant date: Jan 9, 2024
• Expiry date: Feb 20, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.