Patent · US Active

Method for knocking out N-myristoyltransferase (NMT) gene from Eimeria tenella

US11866703B2 · kind B2 · utility

0Cited by

0References

10Claims

0Family size

Assignee

LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

Jianping Cai · Shunde District, CN
Zigang Qu · Lanzhou, CN
Xiao XU · Suzhou, CN
Jing Wang · Taipei, TW
Zhenxing Gong · Lanzhou, CN
Heng Wang · Walnut Creek, US
Xueyang He · Lanzhou, CN
Wenqing Wang · Nanhu, CN

Key dates

Filing date	Dec 23, 2020
Grant date	Jan 9, 2024
Priority date	—
Expiry date	Jul 15, 2041

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2310/20
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present disclosure provides a method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, and belongs to the technical field of microorganisms. The method includes: mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid, and subjecting a resulting mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella. The method provided by the present disclosure can successfully knock out the NMT gene from Eimeria tenella, which lays a foundation for studying the function of the Eimeria tenella gene and developing a vaccine therefor.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.