Patent · US Active

Method for the generation of a cell composition ventral midbrain patterned dopaminergic progenitor cells

US11866729B2 · kind B2 · utility

0Cited by

3References

24Claims

0Family size

Assignee

MILTENYI BIOTEC B.V. & CO. KG · DE

Inventors

Andreas Bosio · Cologne, DE
Andrej Smiyakin · Rösrath, DE

Key dates

Filing date	Sep 3, 2020
Grant date	Jan 9, 2024
Priority date	—
Expiry date	Nov 26, 2041

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2533/52
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention discloses an in vitro method for the generation of a cell composition comprising or consisting of ventral midbrain dopaminergic progenitor cells from a cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps of A) differentiating said pluripotent and/or multipotent stem cells into ventral dopaminergic progenitor cells, thereby generating a cell composition comprising ventral dopaminergic progenitor cells comprising ventral midbrain dopaminergic progenitor cells and ventral hindbrain dopaminergic progenitor cells, and B) Enriching CD117 positive cells from said cell composition comprising ventral dopaminergic progenitor cells by using an antigen binding molecule specific for the CD117 antigen, thereby generating said cell composition comprising or consisting of ventral midbrain dopaminergic progenitor cells. Cell compositions obtainable by said method are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.