Composition for improving molecular barcoding efficiency and use thereof
US11866765B2 · kind B2 · utility
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2References
11Claims
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Key dates
| Filing date | Apr 8, 2021 |
| Grant date | Jan 9, 2024 |
| Priority date | — |
| Expiry date | Jan 1, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to a composition for improving molecular barcoding efficiency and a use thereof, and provided are a method for preparing a DNA library, a molecular barcoding method for nucleic acid sequencing, and a composition for preparing a DNA library. According to the method and composition, by using a barcode sequence arranged to include the position of a first nucleotide in an adapter on the basis of a terminal region of the adapter ligated with a DNA fragment, molecular barcoding switching can be reduced and the accuracy of nucleic acid sequencing can be improved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.