Isolated oligonucleotides containing modified nucleotides
US11866773B2 · kind B2 · utility
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Key dates
| Filing date | Nov 15, 2021 |
| Grant date | Jan 9, 2024 |
| Priority date | — |
| Expiry date | Feb 18, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described and featured herein are isolated oligonucleotides containing, from 5′ to 3′, a nicking enzyme recognition sequence, at least 9 nucleotides that specifically bind a target nucleic acid molecule, and 2′ modified nucleotides. The isolated oligonucleotides may be used in compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a Nicking and Extension Amplification Reaction (NEAR) reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.