Protein biomarker and uses thereof

US11873344B2 · kind B2 · utility

Assignees

• DAIICHI SANKYO COMPANY, LIMITED · JP
• DAIICHI SANKYO EUROPE GMBH · DE
• KINKI UNIVERSITY · JP
• AMGEN INC. · US

Inventors

• Matthias Schneider · Dossenheim, DE
• Sabine BLUM · Munich, DE
• Renee Jeanne MENDELL-HARARY · Skillman, US
• Daniel J. Freeman · Newbury Park, US
• Robert Allen BECKMAN · Blue Bell, US
• Xiaoping Jin · Orange, US
• Kimio YONESAKA · Osakasayama, JP
• Kazuhiko Nakagawa · Osaka, JP

Key dates

• Filing date: Aug 21, 2019
• Grant date: Jan 16, 2024
• Expiry date: Dec 21, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/4756
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention is directed to methods of identifying and treating a human subject harboring a tumor or other disease comprising assessing HRG gene expression at a protein level in the human subject and administering a treatment comprising an anti-HER3 antibody to the human subject whose HRG gene expression at a protein level is assessed as high. The present invention is also directed to methods of identifying a human subject harboring a tumor or other disease comprising assessing HRG gene expression at a protein level in the human subject and withholding a treatment comprising an anti-HER3 antibody to the human subject whose HRG gene expression at a protein level is assessed as low. The invention is also directed to methods of performing an ELISA, including sequential steps of contacting a solid surface with a plurality of solutions each comprising in turn a capture antibody, a blocking agent, a sample suspected of containing an analyte, a detection antibody and an enzyme conjugate, in which the solid surface is subjected to a wash process after each sequential step.