Bacterial cultures and methods for production of alginate
US11873477B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 2019 |
| Grant date | Jan 16, 2024 |
| Priority date | — |
| Expiry date | Mar 14, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/38
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate Oacetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.