Patent · US Active

Sensor for NADP (H) and development of alcohol dehydrogenases

US11875298B2 · kind B2 · utility

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8Claims

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Assignee

Forschungszentrum Jülich GmbH · DE

Inventors

Solvej Siedler · Aachen, DE
Georg Schendzielorz · Goldkronach, DE
Stephan Binder · Eschweiler, DE
Lothar Eggeling · Jülich, DE
Stephanie Bringer-Meyer · Jülich, DE
Michael Bott · Jülich, DE

Key dates

Filing date	Jul 22, 2019
Grant date	Jan 16, 2024
Priority date	—
Expiry date	May 28, 2042

Classification

Technology area (CPC H)Electricity
CPC primaryH04M1/737
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.