Therapeutic protein selection in simulated in vivo conditions
US11879896B2 · kind B2 · utility
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Key dates
| Filing date | Aug 22, 2022 |
| Grant date | Jan 23, 2024 |
| Priority date | — |
| Expiry date | Aug 22, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6854
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining the effect of non-specific interactions in simulated in vivo conditions is presently disclosed. The method includes (a) contacting a solution comprising a biologically relevant molecular crowding agent and a target molecule with a biosensor, wherein the surface of the biosensor comprises a capture molecule that specifically binds the target molecule; (b) allowing the target molecule to bind to the capture molecule; and (c) determining an amount of the target molecule bound to capture molecule using biolayer interferometry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.