Fusion proteins for base editing

US11884947B2 · kind B2 · utility

Assignee

• SHANGHAITECH UNIVERSITY · CN

Inventors

• Jia Chen · Changhua City, TW
• Li Yang · Shanghai, CN
• Xingxu Huang · Shanghai, CN
• Bei Yang · Shiwen, TW
• Xiao Wang · Zürich, CH
• Jianan Li · Fujian, CN

Key dates

• Filing date: Feb 22, 2019
• Grant date: Jan 30, 2024
• Expiry date: May 12, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are fusion proteins that include an apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) and a clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) protein, optionally further with uracil glycosylase inhibitor (UGI). Such a fusion protein is able to conduct base editing in DNA by deaminating cytosine to uracil, even when the cytosine is in a GpC context or is methylated.