Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications

US11885803B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Daniel Takashi Kamei · Monterey Park, US
• Yin To Chiu · Irvine, US
• Benjamin M. Wu · San Marino, US
• Garrett L. Mosley · Anaheim, US

Key dates

• Filing date: Feb 16, 2022
• Grant date: Jan 30, 2024
• Expiry date: Feb 16, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/56983
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens (e.g., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6×106 cells/mL, less than about 3×106 cells/ml, less than about 1×106 CFU/mL, or less than about 50 μg/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component.