Probes, microarray, primers, kit and applications for rapid detection of clinical ophthalmic microorganisms
US11891670B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 6, 2022 |
| Grant date | Feb 6, 2024 |
| Priority date | — |
| Expiry date | Sep 6, 2042 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are probes, primers, a microarray, a kit and applications for rapid detection of clinical microorganism in ophthalmology, belonging to that technical field of clinical microorganism detection. The probes of that disclosure comprise probes for respectively detect Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, Staphylococcus hominis, Serratia marcescens, Escherichia coli, Bacillus subtilis and Enterobacter cloacae. The disclosure also discloses primers for amplifying the target bacteria, which comprises primers which can amplify gene sequence fragments with intra-species homology of more than 95 percent (%) and inter-species homology of less than 75%. The disclosure also provides a method for synthesizing the hybridization probe on the microarray, a method for hybridization reaction and a method for scanning detection. The probes described in the present invention are highly specific and detects microorganisms in ophthalmic clinical samples with sufficient positivity, high accuracy and deliver diagnosis in a short period of time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.