Patent · US Active

Methods for culturing cells and kits and apparatus for same

US11913024B2 · kind B2 · utility

2Cited by

117References

66Claims

0Family size

Assignee

Juno Therapeutics, Inc. · US

Inventors

Lothar GERMEROTH · Oettingen, DE
Christian STEMBERGER · Holzkirchen, DE
Patricia Graf · Munich, DE

Key dates

Filing date	Oct 20, 2016
Grant date	Feb 27, 2024
Priority date	—
Expiry date	Jul 26, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2501/998
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Provided herein are methods for culturing cells, including stimulating or expanding (proliferating), a plurality of cells in a composition of cells such as a population of lymphocytes. In some aspects, provided methods and reagents for the culturing, such as stimulation or expansion (proliferation), of cell populations involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are multimerization reagents and the one or more agents are multimerized by reversibly binding to the reagent. In some aspects, the multimerized agent can provide for expansion or proliferation or other stimulation of a population of cells, and then such stimulatory agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.