Method of preparing mimicking angiogenic co-spheroids and application thereof
US11913030B2 · kind B2 · utility
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3Claims
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Key dates
| Filing date | Sep 5, 2019 |
| Grant date | Feb 27, 2024 |
| Priority date | — |
| Expiry date | Jan 1, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides a method of preparing mimicking angiogenic co-spheroids, including: co-cultring a neural related cell and a cultured cell on hyaluronan-grafted chitosan (CS-HA) substrates to form a co-spheroid of neural related cell/cultured cell, and encapsulating the co-spheroid of neural related cell/cultured cell into a hydrogel to form a mimicking angiogenic co-spheroid. The mimicking angiogenic co-spheroid of the present disclosure can be formed by 3D printing model as a 3D mini-neurovascular unit, which is applicated to a high-throughput angiogenesis screening platform.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.