Patent · US Active

System and method for isolation and qualification of nucleic acids

US11913062B2 · kind B2 · utility

0Cited by

0References

2Claims

0Family size

Assignees

Roche Sequencing Solutions, Inc. · US
Signature Diagnostics GmbH · DE

Inventors

Victoria Constandse · Cape Town, ZA
Daniel Klass · Palo Alto, US
Alexander Lovejoy · Newark, US
Melissa Loyzer · Hamilton, CA
Bronwen Miller · Cape Town, ZA
Bernd Hinzmann · Berlin, DE
Sophie Beckert · Neuzelle, DE

Key dates

Filing date	Nov 18, 2019
Grant date	Feb 27, 2024
Priority date	—
Expiry date	Sep 30, 2042

Classification

Technology area (CPC G)Physics
CPC primaryG16B30/00
WIPO fieldOrganic fine chemistry
WIPO sectorChemistry

Abstract

Present disclosure provides a method including isolating DNA from a source, thereby providing a composition including the isolated DNA. The isolated DNA has at least first and second target regions, where the length of the second target region is greater than the length of the first target region. The method further includes quantifying a total mass of the isolated DNA, quantifying a first quantification cycle (Cq) of the first target region and a second Cq of the second target region, and calculating a Q-ratio for the isolated DNA by dividing the second Cq by the first Cq. The method further includes determining a value for a quality-mass constant (kQm), estimating a required input mass by dividing kQm by the Q-ratio, and preparing the isolated DNA for sequencing if the total mass of the isolated DNA in the composition is equal or greater than the required input mass.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.