Proximity-dependent split RNA polymerases as a versatile biosensor platform
US11913081B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Jun 6, 2017 |
| Grant date | Feb 27, 2024 |
| Priority date | — |
| Expiry date | Jul 9, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/73
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A proximity dependent split T7 RNAP (RNA polymerase) sensor using continuous molecular evolution is described. The versatility of the platform is described by creating robust light and small molecule-responsive genetic sensors. The activity-responsive RNAP platform dramatically simplifies and expands genetic circuit creation, and opens new opportunities in protein engineering, synthetic biology, and bioengineering.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.