Method for identifying DNA base editing by means of cytosine deaminase
US11920151B2 · kind B2 · utility
Assignees
Inventor
Key dates
| Filing date | Sep 13, 2017 |
| Grant date | Mar 5, 2024 |
| Priority date | — |
| Expiry date | Mar 13, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y305/04004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are: a composition for DNA double-strand breaks (DSBs), comprising (1) a cytosine deaminase and an inactivated target-specific endonuclease, (2) a guide RNA, and (3) a uracil-specific excision reagent (USER); a method for producing DNA double-strand breaks by means of a cytosine deaminase using the composition; a method for analyzing a DNA nucleic acid sequence to which base editing has been introduced by means of a cytosine deaminase; and a method for identifying (or measuring or detecting) base editing, base editing efficiency at an on-target site, an off-target site, and/or target specificity by means of a cytosine deaminase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.