Methods for efficient tomato genome editing

US11926835B1 · kind B1 · utility

Assignee

• INARI AGRICULTURE TECHNOLOGY, INC. · US

Inventors

• Tomá{hacek over (s)} {hacek over (C)}ermák · Brookline, US
• Richard Bailey Flavell · Thousand Oaks, US
• Eva Konecna · Brookline, US
• Yajie Niu · Lexington, US
• Michael Lee Nuccio · Salem, US
• Jennifer A. Raji · Waltham, US
• Randall William Shultz · Acton, US
• Davide Sosso · Villaggio Breda, IT
• Maria Margarita D. Unson · Pawcatuck, US
• John Patrick CASEY, JR. · Boston, US
• Michael Andreas Kock · Schifferstadt, DE

Key dates

• Filing date: Jan 29, 2019
• Grant date: Mar 12, 2024
• Expiry date: Jan 29, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure relates to improvements in methods for efficient editing of two or more endogenous tomato genes by insertion of one or more single stranded DNA donor molecules that are not homologous to the insertion sites in the tomato genome.