Method for detecting on-target and predicted off-target genome editing events
US11926866B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Jan 4, 2021 |
| Grant date | Mar 12, 2024 |
| Priority date | — |
| Expiry date | Feb 17, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for detecting on-target and predicted off-target genome editing events by providing a multiplex PCR reaction mixture with an on-target oligonucleotide primer and one or more off-target oligonucleotide primers and then hybridizing the on-target oligonucleotide primer and the one or more off-target oligonucleotide primers to target nucleic acid sequences, followed by cleaving blocking groups from the primers and extending the primers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.