Engineered transaminase polypeptides and uses thereof
US11932886B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 22, 2018 |
| Grant date | Mar 19, 2024 |
| Priority date | — |
| Expiry date | Jun 30, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.