Library-scale engineering of metabolic pathways

US11939571B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• The General Hospital Corporation · US

Inventors

• Xiaoge Guo · Brookline, US
• Alejandro Chavez · New York, US
• Max Schubert · Emeryville, US
• Eric Kelsic · Boston, US

Key dates

• Filing date: Jun 12, 2017
• Grant date: Mar 26, 2024
• Expiry date: Jul 28, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/08
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Expressing guide nucleic acids (e.g., gRNA) from the same oligonucleotide that contains donor sequence permits the high efficiency, simultaneous transformation of a population of cells with both substrates. Using oligonucleotide chip array technology, one can construct thousands of oligonucleotides with customized gRNA and donor sequence in a cost effective manner. In combination, one can efficiently modify endogenous and exogenous genes.