Systems and methods for computing the contributions of autofluorescence in multichannel image

US11941861B2 · kind B2 · utility

Assignee

• Ventana Medical Systems, Inc. · US

Inventors

• Joerg Bredno · San Francisco, US
• Mehrnoush Khojasteh · Tucson, US
• Ilya Ravkin · Palo Alto, US
• Smadar Shiffman · Nes Ziona, IL
• Xingwei Wang · Lo Wu, CN

Key dates

• Filing date: Aug 6, 2021
• Grant date: Mar 26, 2024
• Expiry date: Jul 30, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6441
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein are systems and methods of estimating the autofluorescence (AF) signal and other non-target signals in each channel of a multi-channel image of a biological sample that is stained with one or more fluorescent labels. In some embodiments, the estimated autofluorescence signal can then be subtracted or masked from the multi-channel image. In some embodiments, the autofluorescence-removed multichannel image can then be use for further processing (e.g. image analysis, etc.).