Nerve conduit loaded with adipose-derived stem cells and preparation method thereof

US11944721B2 · kind B2 · utility

Assignee

• Shanghai Sixth People's Hospital · CN

Inventors

• Yun Qian · Doylestown, US
• Cunyi Fan · Suzhou, CN
• Xiangyun Yao · Shanghai, CN
• Zhiwen Yan · Shanghai, CN
• Lingchi Kong · Shanghai, CN
• Xu Wang · 安丰镇, CN

Key dates

• Filing date: Apr 22, 2023
• Grant date: Apr 2, 2024
• Expiry date: Apr 22, 2043

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61L2430/32
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A nerve conduit loaded with adipose-derived stem cells and a preparation method thereof are provided. The preparation method includes: S1, adding polycaprolactone and polyvinylpyrrolidone into a binary organic solvent, performing ultrasonic treatment, and then adding reduced graphene oxide nanoparticles to obtain a spinning solution; S2, electrospinning with the spinning solution and then washing for several times to obtain a semi-finished conduit product; and S3, injecting a cell mixture into the semi-finished conduit product to obtain the nerve conduit. A fiber surface of the nerve conduit has groove structures, and thus a specific surface area and cell adhesion sites are increased, and adhesion and proliferation of cells are facilitated. By loading the adipose-derived stem cells, neurotrophic phenotypic effect of peripheral nerve scaffold is improved, and can effectively avoid immunological rejection of transplantation, promote orientational growth of axons into the nerve conduit and promote myelination effect of Schwann cells.