Bioengineered in vitro 3D model of human atherosclerotic plaque
US11946071B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | Aug 23, 2018 |
| Grant date | Apr 2, 2024 |
| Priority date | — |
| Expiry date | Nov 16, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2513/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method for the generation of a layered cellular 3 D microtissue aggregate, comprising the steps of contacting myeloid cells with a protein kinase C agonist, yielding primed myeloid cells; incubating the primed myeloid cells in the presence of LDL in a confined volume, particularly in a hanging drop culture; yielding a 3 D culture of myeloid cells; and incubating the 3 D culture together with fibroblasts in a hanging drop in the presence of LDL, yielding the layered cellular aggregate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.