Method for non-enzymatic 3D culture and amplification of mesenchymal stem cells
US11946073B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 8, 2022 |
| Grant date | Apr 2, 2024 |
| Priority date | — |
| Expiry date | Jun 7, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2539/10
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for non-enzymatic 3D culture and amplification of mesenchymal stem cells (MSCs) includes the followings steps: preparing PLGA porous microspheres; preparing a PLGA-PEG-PLGA thermosensitive coating microcarrier; culturing and amplifying MSCs; and performing non-enzymatic separation of MSCs, including reducing a culture temperature to below a critical phase transition temperature, and centrifuging a culture medium to collect stem cells. The present invention adopts the method for non-enzymatic 3D culture and amplification of MSCs, wherein the PLGA porous microspheres are used as a cell culture microcarrier scaffold and the thermosensitive hydrogel PLGA-PEG-PLGA is coated on surfaces of such microspheres, without needing additional enzymolysis process, thus efficiently amplifying the stem cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.