Flow cell with integrated manifold
US11951477B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 29, 2022 |
| Grant date | Apr 9, 2024 |
| Priority date | — |
| Expiry date | Jun 29, 2042 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/08
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.