Recombinant foot-and-mouth disease virus with reduced immunosuppression activity, and preparation method and use thereof

US11952401B2 · kind B2 · utility

Assignee

• LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

• Haixue Zheng · Huangyang, CN
• Fan Yang · Brookline, US
• Zixiang Zhu · Lanzhou, CN
• Xiangle Zhang · Lanzhou, CN
• Weijun Cao · Huangyang, CN
• Congcong Wang · Shouguang City, CN
• Kangli Li · Lanzhou, CN
• Huisheng Liu · Laishui, CN
• Hong Tian · Zhengzhou, CN
• Keshan Zhang · Huangyang, CN
• Xiangtao Liu · Lanzhou, CN

Key dates

• Filing date: Jul 14, 2021
• Grant date: Apr 9, 2024
• Expiry date: Feb 15, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/32171
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure belongs to the technical field of biological products for veterinary medicine, and specifically relates to a recombinant foot-and-mouth disease virus (FMDV) with a reduced immunosuppressive activity, a preparation method and use thereof, and a recombinant vaccine strain. According to the present disclosure, it is firstly discovered that FMDV 3B protein has an immunosuppressive function, and key sites for exerting the immunosuppressive function are found. A recombinant FMDV vaccine strain with a lost immunosuppressive function in FMDV 3B protein is constructed by introducing amino acid mutations into three repeated copies of FMDV 3B protein.