Nucleic acid detection or quantification method, chip and assay kit therefor, device for detecting or quantifying nucleic acid and program therefor
US11952620B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 11, 2020 |
| Grant date | Apr 9, 2024 |
| Priority date | — |
| Expiry date | Jul 7, 2041 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/18
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.