Optimization method for capturing proteins by multi-column continuous chromatography (MCC)
US11958881B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 6, 2023 |
| Grant date | Apr 16, 2024 |
| Priority date | — |
| Expiry date | Mar 6, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/22
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
An optimization method for capturing proteins by multi-column continuous chromatography (MCC), including the following steps: step 1, under the conditions of a set loading protein concentration and an arbitrary load residence time, performing a single time of protein breakthrough experiment to obtain a protein breakthrough curve; step 2, under a set breakthrough percentage for a target protein, integrating the breakthrough curve to obtain a single-column loading capacity and establishing a linear relationship between the interconnected load time and the load residence time; step 3, solving for the optimal number of operating columns for capturing proteins by MCC based on step 2; step 4, solving for the optimal load residence time for capturing proteins by MCC based on step 2, step 3; and step 5, solving for the maximum productivity of capturing proteins by MCC based on step 4.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.