Methods and compositions for generating reference maps for nanopore-based polymer analysis
US11959133B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 19, 2020 |
| Grant date | Apr 16, 2024 |
| Priority date | — |
| Expiry date | Feb 5, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/447
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.