Kit and method for constructing long-acting depression animal model

US11963519B2 · kind B2 · utility

Assignee

• Northwest Institute of Plateau Biology, CAS · CN

Inventors

• Cen Li · Missouri City, US
• Hongxia Yang · Beijing, CN
• Jing Zhao · Shanghai, CN
• Lixin Wei · Solo Court, CN
• Yuzhi Du · Solo Court, CN
• Yuancan Xiao · Solo Court, CN
• Hongtao Bi · Solo Court, CN
• Zhanjuan Chen · Solo Court, CN
• Yajun Qiao · Solo Court, CN
• Jianv Wang · Solo Court, CN

Key dates

• Filing date: Aug 25, 2022
• Grant date: Apr 23, 2024
• Expiry date: Aug 25, 2042

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides a kit and method for constructing a long-acting depression animal model. The present disclosure adopts Bacille Calmette-Guerin (BCG) (or Mycobacterium bovis (M bovis)) and low-dose pertussis toxin (PTX) for combined induction, which greatly reduces modeling cost. A mouse model constructed by the method of the present disclosure can clearly distinguish a time interval of pathological behaviors from a time interval of depression-like behaviors and has prominent face validity and predictive validity. The method of the present disclosure is simple and convenient and does not require a large number of tedious daily operations as the depression model construction by chronic unpredictable stress, chronic restraint stress, social defeat, mother-child separation, or the like. The mouse model of the present disclosure can provide a valuable long-term intervention window for research on antidepressant therapy.