Patent · US Active

Methods of associating phenotypical data and single cell sequencing data

US11965208B2 · kind B2 · utility

0Cited by
129References
30Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 15, 2020
Grant dateApr 23, 2024
Priority date—
Expiry dateJul 29, 2040

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/16
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.