Multiplexed on-chip impedance cytometry system and method

US11965810B2 · kind B2 · utility

Assignee

• University of Virginia Patent Foundation · US

Inventors

• Nathan Swami · Charlottesville, US
• John J. McGrath · Kennett Square, US
• Walter Varhue · Colchester, US
• Carlos Honrado · Charlottesville, US
• Vahid Farmehini · Charlottesville, US
• Yi Liu · Qingdao, CN

Key dates

• Filing date: Sep 26, 2019
• Grant date: Apr 23, 2024
• Expiry date: Feb 11, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1028
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.