Device for collecting and culturing fish embryos and method for evaluating combined toxicity of thiamethoxam and tetraconazole

US11969481B2 · kind B2 · utility

Assignee

• ZHEJIANG ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

• Yanhua Wang · Beijing, CN
• SHANSHAN DI · Hangzhou City, CN
• Guiling Yang · Hangzhou City, CN
• Zhiheng Zhang · Nanjing, CN
• Xinquan Wang · Zhejiang, CN
• Qiang Wang · Beijing, CN

Key dates

• Filing date: Jul 31, 2020
• Grant date: Apr 30, 2024
• Expiry date: Aug 24, 2042

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/81
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.