Patent · US Active

Methods of synthesizing oligonucleotides using tethered nucleotides

US11970697B2 · kind B2 · utility

0Cited by

48References

20Claims

0Family size

Assignee

Twist Bioscience Corporation · US

Inventors

Jeremy Lackey · Foster City, US
David Dodd · Los Gatos, US

Key dates

Filing date	Oct 18, 2021
Grant date	Apr 30, 2024
Priority date	—
Expiry date	Oct 18, 2041

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2320/33
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.