Methods and systems for fluorescence detection using infrared dyes
US11971354B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 17, 2019 |
| Grant date | Apr 30, 2024 |
| Priority date | — |
| Expiry date | Sep 26, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6441
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and systems for time-domain and wavelength domain resolved multiplexed time-resolved fluorescence of a sample having at least one of a first fluorescent label bound to a first analyte and a second fluorescent label bound to a second analyte. The sample also includes a third fluorescent label bound to a third analyte and a fourth fluorescent label bound to a fourth analyte. The first and second fluorescent labels emit wavelengths having a first and second lifetime. The third fluorescent label comprises an upconverting phosphor (UCP) emitting at a wavelength having a third lifetime, and the fourth fluorescent label comprises an infrared dye emitting at wavelength having a fourth lifetime. The methods and systems measure as a function of time intensities of at least one of more of the first, second, third, and fourth fluorescent labels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.