Peptide library constructing method
US11978533B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 26, 2017 |
| Grant date | May 7, 2024 |
| Priority date | — |
| Expiry date | Oct 19, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B35/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved peptide library preparation method is described for constructing complete virtual peptide libraries such as a complete virtual tripeptide library, tetrapeptide library, pentapeptide library, hexapeptide library, heptapeptide library, or a complete octapeptide library, etc. The method includes constructing an expression vector for the expression of cyclic peptides. Each cyclic peptide displays an array of peptides of different sizes and sequences, and the number of cyclic peptides needed for constructing a complete virtual peptide library can be dramatically reduced compared with conventional chemical peptide synthesis. Furthermore, the cyclic peptide libraries can be readily reproduced by the expression and purification of the cyclic peptides using the constructed gene libraries. The improved peptide library preparation method can particularly be used, for example, to construct a complete virtual tetrapeptide library, a complete virtual pentapeptide library, a complete virtual hexapeptide library, a complete virtual heptapeptide library, and so on. The improved peptide library preparation method can also be used, for example, to construct a partial pentapeptide library,…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.