Patent · US Active

High-throughput single-cell sequencing with reduced amplification bias

US11981891B2 · kind B2 · utility

0Cited by

45References

24Claims

0Family size

Assignees

Illumina, Inc. · US
University of Washington · US

Inventors

Frank J. Steemers · Encinitas, US
Jay Shendure · Seattle, US
Yi Yin · Munich, DE

Key dates

Filing date	May 17, 2019
Grant date	May 14, 2024
Priority date	—
Expiry date	May 17, 2039

Classification

Technology area (CPC B)Performing Operations; Transporting
CPC primaryB82Y5/00
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the methods include linear amplification of the nucleic acids. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the nucleic acids include three index sequences. Also provided herein are compositions, such as compositions that include the nucleic acids having three index sequences.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.