Patent · US Active

7β-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid

US11981935B2 · kind B2 · utility

0Cited by

1References

8Claims

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Assignee

PHARMAZELL GMBH · DE

Inventors

Dirk Weuster-Botz · Munich, DE
Michael Braun · Heidelberg, DE
Arno Aigner · Bad Aibling, DE
Boqiao Sun · Munich, DE
Christina Kantzow · Munich, DE
Sven Bresch · Munich, DE
Daniel Bakonyi · Cologne, DE
Werner Hummel · Titz, DE

Key dates

Filing date	Jan 15, 2021
Grant date	May 14, 2024
Priority date	—
Expiry date	Apr 16, 2042

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y101/01201
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

In various aspects and embodiments, the invention provides a nucleic acid molecule comprising a nucleotide sequence encoding a 7β-hydroxysteroid dehydrogenase (7β-HSDH) mutant that catalyzes at least the stereospecific enzymatic reduction of a 7-ketosteroid to the corresponding 7-hydroxysteroid, wherein the mutant has, compared to the wildtype 7β-HSDH of SEQ ID NO:2, a decreased substrate inhibition and/or an altered cofactor usage, and the mutant has, in comparison with the wildtype 7β-HSDH of SEQ ID NO:2, 1 to 15 amino acid additions, substitutions, deletions and/or inversions in the sequence motif VMVGRRE corresponding to positions 36 to 42 of SEQ ID NO:2.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.