Cell recovery method and device
US11982601B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 27, 2023 |
| Grant date | May 14, 2024 |
| Priority date | — |
| Expiry date | Feb 27, 2043 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/088
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure provides a method of cell sedimentation and retention of target cells, for example circulating tumour cells, CTC, from a fluid sample onto a solid support. The method comprises placing a fluid medium comprising the target cells in a fluid chamber, the fluid chamber having an open end sealed against a surface of the solid support. The method further comprises subjecting the fluid medium to centrifugation within the fluid chamber to induce sedimentation of the target cells and promote cell adhesion to the surface of the solid support. The method further comprises, post-centrifugation, positioning a fluid absorbing element in the fluid chamber to remove fluid from the fluid chamber. The method further comprises controlling a flow rate of the fluid being absorbed by the fluid absorbing element such that the sedimented cells are not detached from the surface of the solid support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.