Compostable silica encapsulation of oligonucleotides for long-term storage
US11993769B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 14, 2021 |
| Grant date | May 28, 2024 |
| Priority date | — |
| Expiry date | Mar 22, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG11C13/0019
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
This disclosure describes particles and techniques for storing oligonucleotides that provide stable, long-term protection yet are also compostable. A core is coated with a layer of oligonucleotides and encapsulated under an outer layer of non-porous, hydrolyzed organosilicon disulfide. The hydrolyzed organosilicon disulfide protects the oligonucleotides from oxidative and thermal damage under typical storage conditions. One suitable organosilicon disulfide is bis(3-triethoxysilylpropyl) disulfide (BTSPD). The oligonucleotides may be retrieved by contacting the particles with a reducing agent that degrades the disulfide bonds in the outer layer. The disulfide bonds enable removal of the protective encapsulation without the use of dangerous chemicals such as hydrogen fluoride. Instead of retrieving the oligonucleotides, the particles may be disposed of in a composting environment. In an implementation, the oligonucleotides are artificially synthesized and encode digital information.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.