Methods for extending polynucleotides
US11993773B2 · kind B2 · utility
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7Claims
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Key dates
| Filing date | Mar 4, 2021 |
| Grant date | May 28, 2024 |
| Priority date | — |
| Expiry date | Feb 16, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/18
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3′-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.