Multiplexed tissue imaging
US11994452B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 15, 2020 |
| Grant date | May 28, 2024 |
| Priority date | — |
| Expiry date | Nov 8, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/00039
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus attaches to a microscope slide to form an enclosed fluidic chamber with input and output ports, where a tissue or cell sample on the slide can be processed using techniques such as immunohistochemical staining. Flow of reagents within the chamber can be laminar and highly uniform across the sample surface to achieve staining that is free of gradients. The sample can be imaged prior to, during, or after any given processing step. Methods for staining and imaging a sample can be implemented through one or more rounds of labeling, label removal or erasure and imaging. Samples can be imaged many times to achieve very high multiplexing levels. Samples can be placed on an imaging apparatus at various times and removed from the imaging apparatus for certain steps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.