Methods for detection of silver nanoparticles
US11994457B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 27, 2022 |
| Grant date | May 28, 2024 |
| Priority date | — |
| Expiry date | May 19, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1006
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detection of the presence and/or quantities of silver nanoparticles in a specimen is shown. The method includes the steps of a) providing a detection organism suspended in a medium, b) treating the detection organism with zinc ions thus effecting auto-fluorescence therefrom, and then measuring degree of fluorescence of the detection organism suspended medium, c) adding the specimen to the detection organism suspended medium, treating the detection organism therein with the specimen for a period of time, and measuring change of fluorescence of the detection organism-suspended medium over time, d) calculating amount of silver ions intracellularly dissolved from the silver nanoparticles and accumulated in the detection organism in view of the change of fluorescence, and e) extrapolating quantity of silver nanoparticles in the specimen in view of the change of fluorescence and the amount of the intracellularly dissolved silver ions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.