Mammalian lung spheroids and lung spheroid cells and uses thereof

US11998570B2 · kind B2 · utility

Assignee

• North Carolina State University · US

Inventors

• Ke Cheng · San Diego, US
• Eric T. Henry · Raleigh, US
• Jhon Cores · Raleigh, US

Key dates

• Filing date: Oct 15, 2020
• Grant date: Jun 4, 2024
• Expiry date: Nov 22, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/50
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Using lung tissues as a source, mammalian lung progenitor cells lung spheroids and lung spheroid cells (LSCs) were prepared. In one embodiment mammalian LSCs were prepared by (i) culturing mammalian lung tissue explant cells under adherent culture conditions to form a first lung cell outgrowth culture; (ii) culturing the first lung cell outgrowth culture under low-adherence conditions to form lung spheroids; and (iii) culturing the lung spheroids under adherent culture conditions so as to form LSCs. The low-adherence conditions may be a cell culture or suspension. The lung spheroids or LSCs may be formulated into pharmaceutical compositions. Uses such as treatment of lung diseases or diagnostics are also provided. Lung spheroids and LSCs represents a simple and highly reproducible method to generate therapeutic lung cells.