Patent · US Active

Hydroporphyrin beads with narrow fluorescence emissions

US11999885B2 · kind B2 · utility

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28References
11Claims
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Key dates

Filing dateJun 12, 2017
Grant dateJun 4, 2024
Priority date
Expiry dateJun 12, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC09K2211/186
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Provided are fluorescent microparticles and/or nanoparticles that have a polymeric matrix and at least one porphyrinic macrocycle associated therewith. The porphyrinic macrocycles associated with the presently disclosed fluorescent microparticles and/or nanoparticles are in some embodiments selected from the group consisting of porphyrins (including 17,18-didehydrophorbines), chlorins (including phorbines), bacteriochlorins (including bacteriophorbines), and isobacteriochlorins (including isobacteriochlorins containing a fused “E” ring). In some embodiments, the porphyrinic macrocycles have structures selected from Formulas I and H. Also provided are populations made up of a plurality of distinct fluorescent microparticles and/or nanoparticles, methods of making and using the same, sets of separately detectable fluorescent microparticles and/or nanoparticles and methods for preparing and using the same, porphyrinic macrocycle-containing dyads conjugated to particles and methods for using the same to calibrate multi-laser flow cytometers, align and/or calibrate confocal fluorescence microscopes, and/or differentially labeling cells and/or other biomolecules, and biomolecules such as…

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