Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same

US11999948B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Hugh E. Olsen · Santa Cruz, US
• Miten Jain · Santa Cruz, US
• Mark A. Akeson · Santa Cruz, US

Key dates

• Filing date: May 3, 2018
• Grant date: Jun 4, 2024
• Expiry date: Jul 25, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/06
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are methods of producing size-selected nucleic acid libraries. The methods include contacting a nucleic acid sample and a nucleic acid binding reagent including an affinity tag, under conditions in which nucleic acids of less than a desired length are substantially bound to the nucleic acid binding reagent and nucleic acids of the desired length are substantially not bound to the nucleic acid binding reagent. The conditions include the duration of the contacting, the concentration of the nucleic acid binding reagent, or both. The methods further include separating, using the affinity tag, the nucleic acids of less than the desired length bound to the nucleic acid binding reagent from the nucleic acids of the desired length not bound to the nucleic acid binding reagent, to produce a size-selected nucleic acid library. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure, are also provided.