Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells

US11999966B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Karl Martin Hjort · Stanford, US
• Sergio Leal-Ortiz · Hayward, US
• Yuhong Cao · Palo Alto, US
• Chris Rehse · Palo Alto, US
• Andy Kah Ping Tay · Stanford, US
• Nicholas A. Melosh · Menlo Park, US

Key dates

• Filing date: Oct 27, 2020
• Grant date: Jun 4, 2024
• Expiry date: Feb 18, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2535/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.